Journal of Dali University

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Survey on Saposhnikovia divaricata in Herbal Medicine Market Using DNA Barcoding

  

  1. (College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)
  • Received:2017-06-19 Online:2018-02-15 Published:2018-02-15

Abstract:

Objective: To investigate Saposhnikovia divaricata(Fangfeng)in the herbal medicine market, and to provide references
for the quality control and market supervision. Methods: DNA barcoding was using to study the sequence alignment, analysis of
variable sites, genetic distance calculation, and adjacency tree construction of the Saposhnikovia divaricata(Fangfeng)from the herbal
medicine market. Results: The sources of the Saposhnikovia divaricata(Fangfeng)from the herbal medicine market were complicated,
including: Seseli mairei, Pimpinella candolleana, Peucedanum ledebourielloides, and Peucedanum praeruptorum. Conclusion: DNA
barcoding can be used in Saposhnikovia divaricata(Fangfeng)identification, which is a convenient tool for market investigation and
supervision.

Key words: DNA barcoding, Saposhnikovia divaricata, market investigation, ITS2